Table 1. Comparison of cell counting and developmental stage classification (Objective 20X).
Image No. | 1 | 2 | 3 | 4 | 5 | 6 | Total |
---|---|---|---|---|---|---|---|
MC (RBCs) | 1933 | 2381 | 2788 | 2870 | 2821 | 1942 | 14735 |
IBC (RBCs) | 2000 | 2340 | 2810 | 2753 | 2801 | 1972 | 14676 |
Error (RBCs) | 3.35% | 1.72% | 0.79% | 4.10% | 0.71% | 1.55% | 4.00% |
MC (iRBCs) | 103 | 90 | 127 | 131 | 134 | 78 | 663 |
IBC (iRBCs) | 106 | 94 | 126 | 131 | 137 | 78 | 672 |
Error (iRBCs) | 2.91% | 4.44% | 0.78% | 0% | 2.24% | 0% | 1.36% |
MC (Schizont) | 50 | 36 | 70 | 63 | 71 | 25 | 315 |
IBC (Schizont) | 51 | 35 | 72 | 64 | 72 | 25 | 319 |
Error (Schizont) | 2.00% | 2.78% | 2.86% | 1.59% | 1.40% | 0% | 1.27% |
MC (Trophozoite) | 53 | 54 | 57 | 68 | 63 | 53 | 348 |
IBC (Trophozoite) | 55 | 59 | 54 | 67 | 65 | 53 | 353 |
Error (Trophozoite) | 3.77% | 9.26% | 5.26% | 1.47% | 3.17% | 0% | 1.44% |
* MC represents the average value of two manual counting performed in the same image which was analysed by image-based cytometer.
* iRBCs—infected Red Blood Cells.
* IBC indicates the result analyzed using the image-based cytometer.