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. 2017 Jun 8;12(6):e0179174. doi: 10.1371/journal.pone.0179174

Fig 2. Cytotoxic effect of braylin and its modulation of nitric oxide production on macrophages.

Fig 2

Panels A and C: J774 cells (A) or peritoneal exudate macrophages (C) were incubated with vehicle (50% propylene glycol in saline, Ct, control group) or different concentrations of braylin (BRA; 10, 20, 40 or 80 μM) for 72 hours and cell viability was determined by Alamar Blue assay. Gentian violet (GV) was used as positive control. Data are expressed as means ± SEM; n = 9 determinations per group. *Significantly different from the vehicle treated cultures (p < 0.05). ANOVA followed by Tukey´s multiple comparison test. Panels B and D: Concentrations of nitrite were determined in J774 macrophages (B) or peritoneal exudate macrophages (D) treated with vehicle (50% propylene glycol in saline, Ct+, control group), braylin (BRA; 10, 20 or 40 μM) or dexamethasone (Dexa; 40 μM) in the presence of LPS (500 ng/mL) + IFN-γ (5 ng/mL). Cell-free supernatants were collected 24 hours after treatments for nitrite quantification by the Griess method. Ct- shows concentrations of nitrite in unstimulated cells. Data are expressed as means ± SEM; n = 9 determinations per group. *Significantly different from the vehicle treated cultures stimulated with LPS + IFN-γ (p< 0.05). ANOVA followed by Tukey´s multiple comparison test.