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. 2017 Jun 8;12(6):e0179338. doi: 10.1371/journal.pone.0179338

Fig 3. The activity of the AtDRTS1 promoter in apical meristems is controlled by an intragenic region that includes the second intron of the gene.

Fig 3

(A) Schematic representation of the dual reporter constructs used to test the activity of the divergent AtDRTS and AtSFH promoters. The uidA gene coding for the GUS protein is under the control of the AtDRTS promoters whereas the gene encoding the red fluorescent protein eqFP611 is controlled by the AtSFH promoters. (B) IMeter analysis of the introns of the AtDRTS genes revealing high scores of both the first and second intron of AtDRTS1. (C to H) Histochemical localization of GUS activity in transgenic Arabidopsis plants carrying different AtDRTS1 promoter constructs. (C) Localization of GUS accumulation in a one-week-old seedling of the SFH7/DRTS1 lines that reveal the inability of the AtDRTS1 5’ flanking region to drive expression in the RAM, magnified in the inset (C1). (D to H) Localization of GUS activity in lines carrying the SFH7/DRTS1i2 construct that includes the second intron of AtDRTS1. One-week-old (D) and two-week-old (E) seedlings showing strong activity of the AtDRTS1 regulatory region in hydathodes and RAM, as highlighted in the insets (E1) and (E2). The activity of the DRTS1i2 promoter construct is clearly detected also in developing stigmas (F), siliques (G) and mature embryos (H).