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. 2017 Jun 8;13(6):e1006360. doi: 10.1371/journal.ppat.1006360

Fig 2. A subset of HAND-associated miRNAs negatively regulate expression of PEX mRNAs.

Fig 2

HEK293T cells were co-transfected with luciferase reporter plasmids (pMIR-REPORT-Luciferase) containing 3’-UTRs from PEX2 (A), PEX7 (B), PEX11B (C) and PEX13 (D) in forward (5’-3’) or reverse orientations (3’-5’), a transfection control reporter plasmid (pMIR-REPORT-β-gal) and miRNA mimics for miR-500a-5p, miR-34c-3p, miR-93-3p, miR-381-3p and miR-344-3p. After 48 hours, cell lysates were subjected to luciferase and β-gal assays. N = 3. Bars represent standard error of the mean. Key to plasmids: Vec = pMIR-REPORT-Luciferase; KLF4 = pMIR-REPORT-Luciferase with 3’ UTR of KLF4 downstream from luciferase cassette; PEX2 = pMIR-REPORT-Luciferase with 3’ UTR of PEX2 downstream from luciferase cassette; PEX7 = pMIR-REPORT-Luciferase with 3’ UTR of PEX7 downstream from luciferase cassette; PEX11B = pMIR-REPORT-Luciferase with 3’ UTR of PEX11B downstream from luciferase cassette; PEX13 = pMIR-REPORT-Luciferase with 3’ UTR of PEX13 downstream from luciferase cassette. As a positive control, miR-344-3p is shown to downregulate expression of luciferase under the control of the 3’UTR of KLF4 mRNA.