Figure 4.
Losartan (L), amyloidosis, and TGF-β1 expression in A/T mice. (a) Western blot analysis with 6E10 antibody revealed no effect of L on soluble Aβ species in cortex and hippocampus of A/T mice. Actin was used as a reference for loading. (b) Insoluble Aβ1–40 and Aβ1–42 levels in L-treated-A/T mice remained unchanged as assayed in half-brain of cortex and hippocampus by ELISA. (c) The surface area occupied by Aβ plaque load was not changed among groups as determined by thioflavin-S staining of dense-core Aβ plaques in both cortex and hippocampus. Error bars represent SEM (n = 4 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001 for comparison to A/T mice using two-way ANOVA followed by Newman–Keuls post hoc test.