Figure 2.

Acidosis augments MNNG-induced PAR polymer formation. (a) Western blots of PAR polymers from neurons exposed to vehicle (0.1% DMSO) or 25 µM MNNG for 15 min followed by medium with a pH of 7.4 or 6.2. (b) Quantification of PAR optical density from neurons exposed to vehicle or MNNG followed by exposure to medium having a pH of 7.4 or 6.2. Data are normalized by optical density of the neurons exposed to vehicle followed by pH 7.4 medium and optical density of β-tubulin and are expressed as means ± 95% confidence interval (n = 3 vehicle groups; n = 6 MNNG groups); * P < 0.01 from vehicle pH 7.4 treatment; † P < 0.001 from MNNG pH 7.4 treatment.