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. 2016 Jan 1;37(6):2185–2195. doi: 10.1177/0271678X16662042

Figure 2.

Figure 2.

Modulation of [14C]diphenhydramine transport across the rat luminal blood–brain barrier (BBB) measured by in situ rat brain perfusion. (a) Effect of cis-inhibition using clonidine 10 mM or tetraethylammonium (TEA) 20 mM as well as of the increase of the BBB pHi (NH4Cl; 30 mM) on [14C]diphenhydramine transport (Kin; µl s−1 g−1; n = 4–5). (b) Effect of Krebs carbonate perfusion buffer at a pHe of 6.40 or 7.40 on [14C]diphenhydramine brain transport, with (black column; n = 4) or without (white column; n = 4) coperfusion with unlabeled diphenhydramine (DPH; 10 mM). (c) [14C]Diphenhydramine brain transport in the whole right brain hemisphere (RH, n = 6) and five right brain regions including the frontal cortex (FCx), parietal cortex (PCx), occipital cortex (OCx), hippocampus (Hp), and striatum (St), with (black column; n = 4) or without (white column; n = 4) coperfusion with unlabeled diphenhydramine (DPH; 10 mM), measured by in situ rat brain perfusion for 60 s. Data represent means ± SD. ***P < 0.001.