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. 2016 Jan 1;37(6):2208–2223. doi: 10.1177/0271678X16662476

Figure 5.

Figure 5.

CDK5 silencing increases BDNF levels in neurons at four months post-ischemia. (A) BDNF levels were measured in the hippocampus at four months post-ischemia. *Significant differences, p ≤ 0.05; n = 4–6 animals/group. (B) Total protein levels of TRKB (a, b), pCREB (Ser 133) (c), and CREB (d) were determined by Western blotting. Densitometric quantification was determined relative to the loading control (tubulin) and normalized to the internal control (SCRmiR-treated sham rats). The values are the mean ± SEM. *Significant differences, p ≤ 0.05; n = 4–6 animals/group. (C) Immunofluorescence in the ipsilateral CA1 region of the hippocampus (bregma−2.56) at four months post-ischemia is shown for NeuN/BDNF (a) and GFAP/NeuN (b). The photomicrographs were obtained at 40 x. Scale bars=15 µm, n = 4–6 animals/group. CDK5, cyclin-dependent kinase 5; SCR, scrambled RNA sequence; GFP, green fluorescent protein.