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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cancer Clin Oncol. 2016 Nov 11;6(1):12–24. doi: 10.5539/cco.v6n1p12

Figure 3.

Figure 3

Stress differentially affects the number and activation of immune cells in spleens of 4T1 mammary tumor bearing mice Splenocytes were isolated from each group of tumor bearing mice and the percentages of cells expressing CD3+ (A) CD3+CD4+ (B), CD3+CD8+ (C), CD3+CD69+ (D) and CD4+Foxp3+ (E) were assessed using multicolor flow cytometry and FACSDiva software. (F) Splenocyte CD8 to CD4+Foxp3+ Treg ratio. Flow cytometry markers, were compared to controls using a cell means model. * p < 0.05 ** p < 0.01, *** p < 0.001.