Table 2.
Effects of cell type-specific ablation of NG2 on cell and axon biology.
| Control | OPC-NG2ko | My-NG2ko | ||
|---|---|---|---|---|
| Sham-operated animals | ||||
| 1 | Number of Olig2+ cells | 115.27 ± 3.8 | 111.02 ± 5.7 | 117.63 ± 5.4 |
| 2 | Number of Olig2+/PDGF-Rα+ OPCs | 14.55 ± 1.7 | 12.31 ± 2 | 14.46 ± 3.5 |
| 1 week after demyelination | ||||
| 3 | Number of Iba1+ macrophages/ microglia | 117.19 ± 22.3 | 120.1 ± 28.1 | 39.64 ± 19.8** |
| 4 | Number of Olig2+/PDGF-Rα+ OPCs | 42.92 ± 1.5 | 32.4 ± 1.7*** | 23.02 ± 7.2*** |
| 5 | Mitotic index of Olig2+/PDGF-Rα+ OPCs (%) | 22.91 ± 3.5 | 13.49 ± 1.5** | 11.93 ± 2.1*** |
| 6 weeks after demyelination | ||||
| 6 | Number of APC+ oligodendrocytes | 62.14 ± 6.7 | 44.48 ± 8.6* | 43.17 ± 4.1** |
| 7 | Well-myelinated axons | 1056 ± 133 | 480 ± 128** | 499 ± 80** |
| 8 | Myelin thickness (G-value) | 0.745 ± 0.089 | 0.861 ± 0.099*** | 0.87 ± 0.065*** |
| 9 | Number of NF-positive axons | |||
| Small: diameter < 1 μm | 1103 ± 60 | 660 ± 66*** | 812 ± 85** | |
| Medium: diameter = 1-2.5 μm | 201 ± 29 | 135 ± 10* | 118 ± 14* | |
| Large: diameter > 2.5 μm | 18 ± 3 | 6 ± 1** | 8 ± 2* | |
The key parameters for Olig2/PDGFRα-positive OPCs, Iba1-positive macrophages/microglia, and APC-positive oligodendrocytes were quantified by immunolabeling in lesions in control, OPC-NG2ko, and My-NG2ko mice (n = 4) for each group) from 1 to 6 weeks after lysolecithin microinjection into the dorsal spinal cord white matter (lines 1-6). Myelination parameters and axon diameters were determined in toluidine blue-stained semi-thin sections (lines 7-9). G-value = diameter of axon only/diameter of axon + myelin sheath. Cell and axon numbers are determined in volumes of 100,000 μm3. Values represent means ± SD. Statistically significant values evaluated by ANOVA and t-test are indicated by
P < 0.05,
P < 0.01,
P < 0.001.
(Reproduced with permission from Kucharova, K. and Stallcup, W. B. 2015, J. Neuroinflamm., 12, 161.)