(
A,B) Lipid and content mixing assays performed under the same conditions as those shown in
Figure 4E,F but in the absence of the synaptotagmin-1 C
2AB fragment. The gray curves show a reference experiment performed in the presence of NSF-αSNAP, Munc13-1 C
1C
2BMUNC
2C and WT Munc18-1. (
C) Quantification of content mixing in the experiments shown in
Figure 4F. Bars represent averages of the normalized fluorescence observed after 800 s (500 s after Ca
2+ addition) in experiments performed at least in triplicate. Error bars represent standard deviations.