FIGURE 1.

An augmented alloimmune response, but not an augmented antiviral response, promotes PyV-associated allograft injury. A, Experimental model for antidonor and antiviral adoptive T cell transfer in the splenectomized aly/aly mouse. C3B6F1 donor kidneys were transplanted into B6 aly/aly recipients with splenectomy and bilateral nephrectomy. Mice were infected by MPyV on day 1 posttransplantation. T cells were transferred on days −2 or −1 pretransplantation. B, Survival of transplanted mice receiving no cells (n = 5), anti-allo cells (n = 6), or antiviral cells (n = 8). C, Serum creatinine at day 60 or time of death and (D) viral load in kidneys at day 60 or time of death. (The number of data points per group is decreased from the initial survival curves due to mouse death before samples could be obtained). Dots represent individual mice. Dashed lines indicate limits of detection. E, Representative histology at day 60 or time or death. Although the transfer of antiviral T cell shows expected infiltration, the transfer of anti-allo T cells shows the greatest degree of histologic damage (H&E staining; original magnification, ×400).