(A) HK2 is highly expressed in NSCLC cells. Western blot analysis was performed to examine HK2 expression in lung cancer cells and normal HBE lung cell, long time exposure, 5 min, short time exposure, 20 sec. (B) HK2 is highly expressed in lung cancer tissues. Immunohistochemical staining was performed on a lung cancer tissue array using a HK2 antibody. The intensity was evaluated using Image-Pro PLUS (v.6) and Image J (NIH) computer software. Statistical analyses were performed using Prism 5.0. The density of each individual sample is shown (right). The asterisk indicates a significant difference (***p < 0.001, Mann-Whitney U-test) between tumor and adjacent tissue as indicated.