Table 2. II Round JAK2V617F mutation burden and summary statistics (minimum, maximum, calculation of median, coefficient of variation) of results obtained by the ten participating laboratories with ipsogen JAK2 MutaQuant kit.
| Sample | Expected JAK2V617FAB (%) | Min. | Median | Max | CV (Coefficient of Variation) |
|---|---|---|---|---|---|
| DNA G | 0 | 0.002 | 0.008234 | 0.02 | 0.73 |
| DNA E | 0.1 | 0.03 | 0.09874 | 0.17 | 0.42 |
| DNA C | 1 | 0.39 | 0.8471 | 1.12 | 0.24 |
| DNA B | 5 | 2.38 | 3.643 | 4.66 | 0.18 |
| DNA F | 10 | 6.04 | 8.794 | 17.65 | 0.38 |
| DNA H | 20 | 13.12 | 15.2 | 19.35 | 0.11 |
| DNA D | 50 | 30.54 | 42.62 | 46.76 | 0.12 |
| DNA A | 100 | 99.76 | 99.92 | 99.97 | 0.0005 |
The expected JAK2V617F mutation burden of the eight DNA samples was 0, 0.1, 1, 10, 20, 50 and 100%.