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. 2017 Mar 14;8(20):32706–32721. doi: 10.18632/oncotarget.16188

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Copyright: © 2017 Karsch-Bluman et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with DAPI (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). (B) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. (C) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in Supplementary Figure 7 (D) Flow cytometry analysis of LLC cells incubated with for 1 hour. Fluorescent detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.