Figure 4. Comparison of SIRT1 activity in mammary glands of COP vs F344 rats after exposure to NMU.

Both F344 and COP rats (3 per group) were exposed to NMU or saline (control) and maintained on control diet, while an additional three NMU-treated F344 rats were maintained on an MSC-enriched diet (3 ppm Se). After 30 days, animals were sacrificed at ZT12 (after lights-off at 6 PM) and total protein was extracted from harvested mammary glands. Protein deacetylase activity was determined with the SIRT1 Fluorimetric Drug Discovery Kit. Enzyme activities were normalized to protein concentration. Significance was evaluated and presented at p<0.05 or p<0.01(one-way ANOVA, n=3).