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. 2017 Mar 2;8(20):32821–32832. doi: 10.18632/oncotarget.15826

Figure 5. Change of DBCCR1 expression in A549 cells results in reduced counter-trend change of methylation.

Figure 5

(A) The mRNA levels of DNMT1 were high-rich in 12 representative lung cancer patient tissues compared with adjacent non-tumor tissues by PCR (p<0.01). (B) The protein levels of DNMT1 were high-rich in A549 compared BEAS-2B by WB. Quantitation by densitometry was shown on below (**P<0.01, compared with non-tumor tissues). A549 cells were transfected with DBCCR1-shRNA or control-shRNA for 48 h for knockdown of DBCCR1. A549 cells were infected with Lenti-virus with DBCCR1 or Lenti-virus control for 48 h for over-expression of DBCCR1. DNMT1 expression was detected by PCR (C) and Western blot (D). Quantitation by densitometry was shown on below (**P<0.01, compared with normal cell line).