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. 2017 Mar 2;8(20):32884–32904. doi: 10.18632/oncotarget.15862

Figure 8. Combinatorial treatment of A549 with polyI:C+anti-IL6 or polyI:C+inhibitors (Stattic /AG490) enhanced polyI:C-killing and suppressed cell motility, migration and invasion.

Figure 8

Scratch wound assay to assess cellular motility and wound closure rate of A549 cells treated for different time intervals (0, 12, 24 h) with: (A) polyI:C+anti-IL6 antibody; (B) polyI:C+Stattic; (C) polyI:C+AG490. Wound closure rate and wound width were measured using ImageJ software. Wound width is presented as percent polyI:C-treated cells +/− combinatorial treatment relative to NT. The representative microscopy images of the wounded areas were examined under 40× magnification. (D) Migration and (E) Invasion were measured after 24-h treatment of A549 cells with polyI:C+anti-IL6 antibody or polyI:C+Stattic or polyI:C+AG490. The migrated or invaded cells underneath the transwell insert were stained by Hoechst 33342, and counted under fluorescence microscopy. The representative images of migrated cells were examined under ×40 magnification. The migrated and invaded cells are presented as percent of cells treated with a combinatorial treatment relative to control NT cells. Bar, 100 μM; *P<0.05, **P<0.01, ***P<0.001 indicate polyI:C-treated cells vs. untreated cells. n.s, non-significant; #P<0.05, ##P<0.01, ###P<0.001 indicate cells treated with combinatorial vs. no combinatorial treatment.