Mice were treated with 2.5% DSS in their drinking water for 7 days to induce acute colitis. Every 2 days, each mouse was given 100 μl solution containing 50 mg Huaier extract intragastrically beginning 1 day before DSS administration. (A) Body weight and (B) Clinical disease activity index were daily observed during the disease process (A: n = 8 in control group and Huaier group; n = 8 in DSS group and DSS + Huaier group, one-way ANOVA; B: n = 8 in DSS group and DSS + Huaier group, Student t-test;). (C) Bleeding score and (D) Stool score of the DSS and DSS+Huaier groups were analyzed during the disease process. Student's t-test, *p < 0.05,**p < 0.01. Data are representative of three experiments (mean and s.d. of triplicate samples).