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. 2017 Mar 23;8(20):32937–32945. doi: 10.18632/oncotarget.16513

Figure 3. Huaier aqueous extract inhibits IL-1β secretion.

Figure 3

(A) Immunohistochemistry of NLRP3 and IL-1β in colonic tissue section. Positive staining is brown. (B) Mouse peritoneal macrophages were pretreated with increasing concentration (0, 4, 8, and 16 mM) of Huaier for 2 h and then primed with LPS (100 ng/ml) for 8 h and stimulated with ATP (2 mM) for 30 min. IL-1β expression in the culture supernatants was determined by ELISA. (C) Mouse peritoneal macrophages were pretreated with PBS or Huaier (8 mM) for 2 h and then stimulated as in (B). TNF-α and IL-6 expression in the culture supernatants were determined by ELISA. Student t-test, **p < 0.01.▲, no significant differences. Data are representative of three experiments (mean and s.d. of triplicate samples).