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. 2017 Mar 23;8(20):32937–32945. doi: 10.18632/oncotarget.16513

Figure 5. Huaier inhibits NLRP3 protein expression.

Figure 5

Mouse peritoneal macrophages were pretreated with PBS or Huaier (8 mM) for 2 h and then stimulated with LPS (100 ng/ml) for indicated time periods. NLRP3 expression was detected by Western blot (A) or RT-PCR (C). Mouse peritoneal macrophages were pretreated with increasing concentration of Huaier (0, 4, 8, 16 mM for B, and 0, 2, 4, 8, 16 mM for D) for 2 h and then stimulated with LPS (100 ng/ml) for 4 h. NLRP3 expression was detected by Western blot (B) or RT-PCR (D). RAW264.7 (E) and THP-1 cells (F) were pretreated with PBS or Huaier (0, 2, 4, 8, 16 mM for E, and 0, 4, 8, 16 mM for F) for 2 h and then stimulated with LPS for 4 h. NLRP3 expression was detected by Western blot. Similar results were obtained in three independent experiments.