Skip to main content
. 2017 Mar 23;8(20):32937–32945. doi: 10.18632/oncotarget.16513

Figure 6. Huaier promotes NLRP3 degradation via promoting autophagy.

Figure 6

(A) Mouse peritoneal macrophages were pretreated with Huaier (8 mM) for 2 h, then stimulated with LPS for 4 h, and subsequently treated for various times with cycloheximide (CHX) (10 μM). NLRP3 expression levels were detected by Western blot. (B, C) Mouse peritoneal macrophages were pretreated with Huaier (8 mM) for 2 h and then stimulated with LPS (100 ng/ml) for 8 h. The cells were treated with MG132 (10μM), chloroquine (100 μM) (B) and 3-MA (10 mM)(C) for the last 4 h. NLRP3 expression was detected by Western blot. (D) Mouse peritoneal macrophages were pretreated with Huaier (8 mM) for 2 h, primed with LPS (100 ng/ml) for 4 h, treated with chloroquine (100 μM) and finally stimulated with ATP (2 mM) for 30 min. IL-1β expression in the culture supernatants was determined by ELISA. (E) Mouse peritoneal macrophages were pretreated with increasing concentration of Huaier (0, 2, 4, 8, and 16 mM) for 2 h and then stimulated with LPS (100 ng/ml) for 8 h. LC3B and NLRP3 were detected by Western blot. Similar results were obtained in three independent experiments.