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GBM28, SJ-GBM2 and U87MG cells were treated with penfluridol (5 μM) for 48 h after (A–B) GBM28 and SJ-GBM2 cells were pretreated with 50 μM LY294002 (C–E) GBM28, U87MG and SJ-GBM2 cells were transfected with AktsiRNA. Levels of pAkt(Ser 473), GLI1, Sox2, Cl caspase3 as well as Cl PARP were evaluated by western blotting.
