Figure 1. Iodide uptake in PDAC cell explants in vitro.

Kras;p53 mice develop PDAC that occupies a large portion of the abdominal cavity below the stomach (A). (B) PDAC cell explants from three separate mice (three technical replicates per mouse) transfected in vitro with LPEI-PEG-GE11/NIS showed a significant increase in perchlorate- (ClO₄⁻-) sensitive ¹²⁵I accumulation compared to transfection with LPEI-PEG-Cys/NIS (mean ± S.E.M.; *p<0.05; **p<0.01; ***p<0.001). No iodide uptake above background levels was observed in cells transfected with LPEI-PEG-GE11 alone. (C) Transfection of EGFR-ablated PDAC cell explants from two mice (three technical replicates per mouse) with LPEI-PEG-GE11/NIS and LPEI-PEG-Cys/NIS showed no significant differences between transfection with targeted or untargeted polyplexes, demonstrating EGFR-specificity of the targeting ligand GE11 (mean ± S.E.M.).