Figure 6. miR-217 and DNMT1 expression in vivo.

(A) miR-217 levels were detected by RT-qPCR. miR-217 expression was significantly upregulated in old skin tissues compared with that observed in young skin tissues (n = 10, *p < 0.05). (B) miR-217 levels were detected by RT-qPCR. miR-217 expression was significantly upregulated in old-aged HSFs compared with that observed in young-aged HSFs (n = 10, *p < 0.05). (C) DNMT1 expression was detected by western blotting. DNMT1 expression in old skin tissues was significantly lower than that observed in young skin tissues (n = 10, *p < 0.05). DNMT1 expression in 4 representative couples is shown (Y: young skin tissues; O: old skin tissues). (D) DNMT1 expression was detected by western blotting. DNMT1 expression in old-aged HSFs was significantly repressed compared with that measured in young-aged HSFs (n = 10, *p < 0.05). DNMT1 expression of 4 representative couples is shown (Y: young-aged HSFs; O: old-aged HSFs). (E) miR-217 levels were negatively correlated with DNMT1 protein levels in different couples of young and old skin tissues (n = 10, R² = 0.532, *p < 0.05). (F) miR-217 levels were negatively correlated with DNMT1 protein levels in different couples of young-aged and old-aged HSFs (n = 10, R² = 0.700, *p < 0.05).