Table 2. Immunohistochemical and molecular findings for 27 patients with non-CD5-T-cell marker-positive B-cell lymphoma.
| Case No. | Histology | Flow cytometry/Immunohistochemistry* | Cell of origin | Light chain | MYC/BCL2 | EBER-ISH | IGH/TCR | FISH split assay | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD2 | CD3 | CD4 | CD5 | CD7 | CD8 | CD8β† | CD20 | CD10 | BCL2 | BCL6 | MYC | |||||||
| 1 | DLBCL | − | − | − | ED/+ | EN/+ | ED/+ | + | +/+ | −/− | GC | K | −/+ | − | NA/NA | − | − | − |
| 2 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | −/− | non-GC | K | −/+ | − | +/− | − | − | − |
| 3 | DLBCL | − | − | − | DN/I | − | ED/+ | − | +/+ | −/− | non-GC | K | −/+ | − | NA/NA | − | − | − |
| 4 | DLBCL | − | − | − | DN/+ | ED/+ | ED/+ | − | +/+ | −/− | non-GC | Λ | +/+ | − | NA/NA | − | + | − |
| 5 | DLBCL§ | − | − | − | −/− | − | ED/+ | − | +/+ | −/− | non-GC | K | −/+ | − | +/NA | − | − | − |
| 6 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | +/+ | GC | Λ | −/+ | − | NA/NA | − | − | − |
| 7 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | D/+ | GC | Λ | +/− | − | NA/NA | − | − | − |
| 8 | IVLBCL | − | − | − | E/+ | − | D/− | − | +/+ | −/− | non-GC | K | −/− | − | NA/NA | − | + | NA |
| 9 | DLBCL | − | − | − | D/+ | DN/+ | DN/+ | − | +/+ | +/+ | GC | Λ | −/+ | − | NA/NA | − | − | − |
| 10 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | +/+ | GC | Λ | −/+ | − | +/− | + | + | − |
| 11 | DLBCL | − | − | − | −/− | − | ED/+ | + | +/+ | +/+ | GC | K | +/+ | − | +/− | + | − | + |
| 12 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | +/+ | GC | Λ | −/+ | − | +/− | − | + | − |
| 13 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | +/+ | GC | K | −/+ | − | NA/NA | − | − | − |
| 14 | DLBCL | − | − | − | −/− | − | D/+ | − | +/+ | +/+ | GC | K | NA/+ | − | NA/NA | − | − | − |
| 15 | DLBCL | − | − | − | −/− | − | DN/+ | − | +/+ | −/− | non-GC | K | −/+ | − | +/− | − | − | NA |
| 16 | DLBCL | − | − | − | −/− | − | E/+ | + | +/+ | −/− | non-GC | Λ | +/+ | − | +/− | − | − | − |
| 17 | DLBCL | − | − | − | −/− | − | E/+ | − | +/+ | −/− | non-GC | K | −/+ | − | NA/NA | − | − | − |
| 18 | DLBCL | − | − | − | −/− | E/+ | D/− | − | +/+ | −/− | non-GC | K | −/+ | − | +/NA | − | − | − |
| 19 | DLBCL | − | − | − | −/− | D/+ | − | NP | +/+ | −/− | non-GC | K | +/+ | − | NA/NA | − | − | − |
| 20 | DLBCL | − | − | − | −/− | E/+ | − | NP | +/+ | −/− | non-GC | K | +/+ | − | NA/NA | − | − | + |
| 21 | DLBCL | − | − | − | −/− | DN/+ | − | NP | +/+ | −/− | non-GC | Λ | −/+ | − | +/− | − | + | − |
| 22 | DLBCL | − | − | − | −/− | DN/I | − | NP | +/+ | +/+ | GC | K | +/+ | − | +/− | − | + | + |
| 23 | DLBCL | − | − | − | −/− | D/+ | − | NP | +/+ | −/− | non-GC | −¶ | +/+ | − | NA/NA | − | − | − |
| 24 | DLBCL | − | − | − | DN/+ | D/+ | − | NP | +/+ | −/− | non-GC | K | −/+ | − | NA/NA | NA | NA | − |
| 25 | DLBCL | D/I | − | − | −/− | − | − | NP | +/+ | −/− | non-GC | Λ | −/− | − | NA/NA | − | − | − |
| 26 | DLBCL | D/+ | − | − | −/− | − | − | NP | +/+ | −/− | GC | Λ | −/− | − | NA/NA | − | − | − |
| 27 | IVLBCL | − | − | D/I | +/+ | − | − | NP | +/+ | −/− | non-GC | Λ | +/+ | − | +/− | − | + | − |
§Case 5 is a patient with DLBCL of the colon associated with an FL grade 3B component in the tonsil. *For T-cell markers other than CD5, the results of immunohistochemistry are given for only flow cytometry positive cases; †only the results of immunohistochemistry are given; ¶absence of expression for both kappa and lambda; IGH, immunoglobulin heavy chain gene rearrangement by southern blot analysis; TCR, T-cell receptor β chain gene rearrangement by southern blot analysis; NP, not performed; NA, not available; E, equal; ED, equal to dim; EN, equal to negative; D, dim; DN, dim to negative; I, indeterminate.