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. 2017 Apr 4;8(20):33501–33514. doi: 10.18632/oncotarget.16819

Figure 2. IL-35 switches the function of neutrophils toward tumor-promoting.

Figure 2

(A) H22 or B16 cells were inoculated into naive mice, pUN01-mice, and pIL-35-mice. CXCL1 was expressed in local tissues to recruit neutrophils, as described in Methods. The effect of neutrophil recruitment on tumor growth was evaluated by measuring tumor weight. (B and C) Neutrophils were isolated from bone marrow (BM) or peritoneal cavity (PC) of naive mice, or pUN01-mice and pIL-35-mice. The neutrophils from BM (B) or PC (C) were used for co-inoculation test as described in Methods. Tumors (n = 9 per group) were dissected and weighed on d10 after tumor cell inoculation. *p < 0.05, **p < 0.01, ***p < 0.001.