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. 2017 Apr 4;8(20):33571–33585. doi: 10.18632/oncotarget.16827

Figure 1. Screening of first- and second-generation Ang2-BD libraries against soluble Tie2.

Figure 1

Shown is a FACS analysis of yeast expressing Ang2-BD. (A) Negative control. (B) Ang2-BDWT expression and binding of Tie2 (10 nM). (C) Ang2-BD library expression and binding of Tie2 (10 nM). (D) Ang2-BD library expression and binding of Tie2 (10 nM) after five rounds of sorting. (E) Ang2-BDC1.70 expression and binding of Tie2 (5 nM). (F) Ang2-BDC1.70 library sort expression and binding of Tie2 (5 nM). (G–I) Ang2-BDC1.70 library expression and binding of Tie2 (5 nM) after sorts 1, 3 and 5, respectively. Sorts 2–5 were conducted using gates similar to the one shown in panel F.