Figure 3. mTOR expression in cholangiocarcinoma cells and the TargetScan result.
(A) TargetScan predicted mTOR was the target gene of miR-199a-3p: miR-199a-3p could bind the 129–135 positions of the mTOR 3′-UTR. (B) Western blotting detected the expression of mTOR and p-mTOR (sites 2481 and 2448) in GBC-SD and RBE cell lines; the results were quantified using ImageJ software (**P < 0.01, ***P < 0.001).