Table 1.
Kinetics Analysis of ENG-BMP9 Interaction by BLI
| KD (M) | kon (M−1s−1) | koff (s−1) | |
|---|---|---|---|
| ECTO | 9.81 × 10−9 | 2.0 × 105 ± 1.69 × 103 | 1.98 × 10−3 ± 1.15 × 10−5 |
| OR | 2.95 × 10−7 | 2.95 × 105 ± 8.96 × 103 | 8.71 × 10−2 ± 9.08 × 10−4 |
| MOR | 1.79 × 10−7 | 3.49 × 105 ± 1.3 × 104 | 6.22 × 10−2 ± 7.76 × 10−4 |
| OR F282V | NBD | ||
| OR S278P | NBD | ||
| OR Q270A/I271A | NBD | ||
| mMBP | NBD |
NBD, no binding detected under the tested experimental conditions. See also Figure S8.