Fig. 3.
SEC analysis of DmHsp27 WT. Size exclusion chromatography (SEC) analysis using a Superose 6 10/300 GL (GE Life Sciences) column, which was calibrated by using IGM (900 kDa), thyroglobulin (669 kDa), ferritin (440 kDa), aldolase (158 kDa), conalbumin (75 kDa), and ovalbumin (43 kDa) to generate a calibration curve. Blue Dextran (2000 kDa) was used to determine the void volume of the column V 0. a Profile on column of 600 μg (dashed line), 300 μg (gray line), and 100 μg (black line) of pure DmHsp27W. Insert (i) present SDS electrophoresis of samples collected (500 μl for each fraction) after size exclusion chromatography. The proteins were detected by Coomassie blue staining. The elution volume is indicated above the gel