Fig. 1.

Primary structure and sequence homology of DmHsp22. a Amino acid sequence alignment of ACDs of DmHsp22 (P02515) and DmHsp27 (P02518) and human HspB1 (P04792), HspB4 (P02489), and HspB5 (P02511) were performed using multiple sequence alignment program (Clustal W). Block columns represent completely conserved amino acid residues. b Twenty-five micrograms of each single arginine mutant in addition to the recombinant DmHsp22WT protein were loaded on 12% SDS-PAGE. M protein molecular weight marker. Proteins were visualized by using Coomassie Brilliant Blue staining G250