Fig. 5.

Novex™ 4–12% Tris-glycine native gel analysis. a Twenty-five micrograms of DmHsp22WT and its arginine mutants after Bradford protein assay were loaded on 4–12% Tris-glycine native gel at 4 °C to compare the oligomeric profiles of proteins. b Native gel analysis of the human R140G-HspB1 versus HspB1WT. Twenty-five micrograms of each purified protein were loaded on 4–12% Tris-glycine native gel and separated either at 4 °C. Proteins were visualized by using Coomassie brilliant blue staining G250