Fig. 2.

Assembly buffers influence CRYAB binding to desmin filaments. The binding of WT desmin to CRYAB was assessed in a) “Phosphate-buffer”, (b) “Tris-buffer” and (c) “Imidazole-buffer” by co-sedimentation assay. CRYAB is present in the supernatant and is also present in the sucrose fractions (1, 2, 6) where desmin is also found. Desmin filaments were found in the insoluble pellet fractions for all three buffer systems. Both proteins were detected in all of the sucrose fractions and were partially found in the pellet in the “phosphate-buffer” and “Tris-buffer”. In contrast, CRYAB was only recovered together with desmin in the insoluble pellet fraction in the imidazole buffer system