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. 2017 Jun 8;7:3056. doi: 10.1038/s41598-017-03435-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The special expression profiles for several tissues of PvNACs. (a–e) Special expression patterns of PvNACs in root, lignified, leave, flower, and seed tissues. AP13_ Seed0d, AP13_ Seed5d, AP13_ Seed10d, AP13_ Seed15d, AP13_ Seed20d, AP13_ Seed25d, AP13_ Seed30d represent whole flowers at anthesis stage, whole seeds 5 days post fertilization, whole seeds with visible caryopsis, whole seeds at the milk stage, whole seeds at the soft dough stage, whole seeds at the hard dough stage, whole seeds at the physiological maturity stage, respectively. Inflo-meristem: Inflorescence meristem (0.5–3.0 mm). Inflo-floret: Floret of inflorescence when glumes are 10–20 mm. Inflo-REL: Rachis and branch elongation of inflorescence (50–150 mm). Inflo-PEM: Panicle emergence of inflorescence (>200 mm). E4-LFB: Pooled leaf blade from plant. E4-LSH: Pooled leaf sheath. E4i3m: Middle 1/5 fragment of the 3rd internode. E4i3mVB: Vascular bundle isolated from 1/5 fragment of the 3rd internode. E4i4b: Bottom 1/5 fragment of the 4th internode. E4i4t: Top 1/5 fragment of the 4th internode. E4i4m: Middle 1/5 fragment of the 4th internode 4. E4-root: Whole root system. E4-crown: Whole crown. E4-node: Pooled nodes. The genes with red frames were chosen for qRT-PCR in further step.