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. 2017 Jun 8;7:3092. doi: 10.1038/s41598-017-03293-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between microfluidic counts and conventional fluorescence microscopic counts of L. pneumophila stained with a fluorescent antibody. Cultured L. pneumophila cells were spiked in cooling tower water. Error bars indicate the standard deviation (n = 5). Samples with 10¹ cells/ml of Legionella cells were counted following 1000-fold concentration by filtration. Samples with 10² and 10³ cells/ml of Legionella were 100-fold concentrated before counting, and samples with 10⁴ to 10⁶ cells/ml of Legionella were counted without concentration.