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. 2017 Jun 8;7:3063. doi: 10.1038/s41598-017-03544-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protection of T2 generation transgenic maize accumulating AfIP-1A/1B in field tests against WCR. (A) Comparison of node-injury scores (root ratings) among treatments across three field locations with high corn rootworm pressure in 2012. Injury from corn rootworm larval feeding was visually assessed using the Iowa State 0–3 Node-Injury Scale²⁰. Estimated node-injury ratings followed by the same letter were not significantly different. A difference was considered statistically significant if the p-value of the difference was less than 0.05. (B) Root protection of a subset of the AfIP-1A/1B plants showing efficacy similar to the commercial DAS-59122-7 control while the non-transgenic negative control reveals significant feeding injury (red arrows). (C) Western analysis of AfIP-1A and AfIP-1B accumulation in roots of nine siblings from Event A.