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. 2017 Jun 8;7:3063. doi: 10.1038/s41598-017-03544-9

Figure 2.

Figure 2

Shared binding sites between AfIP-1A/AfIP-1B and Cry34/35 on BBMVs from WCR midgut tissue. (A) Image of in-gel fluorescence after incubation of BBMVs (10 µg) with Alexa-AfIP-1B (5 nM) with AfIP-1A (10 nM), in the absence or presence of an excess of Cry34/35 or AfIP-1B. (C) Image of gel representing reciprocal heterologous competition assay of Alexa-Cry35Ab1 (0.07 µM) with Cry34Ab1 (10 µM), in the absence or presence of an excess of Cry34/35 or AfIP-1B. Normalized specific binding of AfIP-1B (B) and Cry35Ab1 (D) based on optical densitometry of gel images represented in (A) and (C) after subtraction of nonspecific binding (see Supplementary Information). The data presented are the average and SEM of 3 experiments consisting of 2 or 3 determinations each.