Skip to main content
. 2005 Feb 1;19(3):328–338. doi: 10.1101/gad.1270605

Figure 3.

Figure 3.

Expression and response to phosphosugar stress of the variant ptsG mRNAs. (A) AS16 harboring the Pbla-ptsG2 plasmid pTH121 (lanes 1,2) or the Pbla-ptsG1 plasmid pTH111 (lanes 3,4) were grown with 1% Glc (lanes 1,3) or αMG (lanes 2,4) to A600 = 0.6. Total proteins were prepared and samples equivalent to 0.0075 A600 units were subjected to Western blot analysis using anti-IIB antibody. (B) Cells were grown to A600 = 0.5. Glc (lanes 1,3) or αMG (lanes 2,4) was added at a final concentration of 1%, and incubation was continued for 20 min. Total RNAs were prepared and 3 μg of RNA samples was subjected to Northern blot analysis using the ptsG probe.