Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Relevant genotype and property | Source |
|---|---|---|
| Strain | ||
| IT1568 | W3110 mlc | Wild type |
| AS13 | ΔptsG::cat | This study |
| AS16 | ΔptsG | This study |
| TM224 | Δpgi | Morita et al. 2004 |
| AS17 | Δpgi ΔptsG::cat | This study |
| AS18 | ΔptsG Δpgi | This study |
| AS20 | ΔptsG Δpgi ams-1 zce726::Tn10 | This study |
| TM122 | hfq::cat | This study |
| AS21 | ΔptsG hfq::cat | This study |
| TM540 | Δ(sgrR-sgrS)::cat | This study |
| TM542 | Δ(sgrR-sgrS) | This study |
| AS22 | ΔptsG Δ(sgrR-sgrS)::cat | This study |
| AS23 | ΔptsG Δ(sgrR-sgrS) | This study |
| Plasmid | ||
| pIT499 | Derivative of pSTV28 carrying the intact ptsG | Kimata et al. 1997 |
| pTH111 | Derivative of pBR322 carrying the Pbla-ptsG1 | Takahashi et al. 1998 |
| pTH121 | Derivative of pBR322 carrying the Pbla-ptsG2 | This study |
| pHA7MK | Derivative of pBR322 carrying the Pbla-crp | This study |
| pMM10 | Derivative of pBR322 carrying the ptsG14-crp | This study |
| pMM11 | Derivative of pBR322 carrying the ptsG40-crp | This study |
| pMM12 | Derivative of pBR322 carrying the ptsG72-crp | This study |
| pMM13 | Derivative of pBR322 carrying the ptsG107-crp | This study |
| pMM14 | Derivative of pBR322 carrying the ptsG139-crp | This study |
| pMM12FS | Derivative of pBR322 carrying the ptsG72FS-crp | This study |
| pMM10Y | Derivative of pBR322 carrying the ptsG14Y-crp | This study |
| pMM12FSM | Derivative of pBR322 carrying the ptsG72FSM-crp | This study |