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. 2005 Feb 1;19(3):362–375. doi: 10.1101/gad.1240705

Figure 7.

Figure 7.

Ectopic expression of Hic-5 in 3T3-L1 preadipocytes inhibits adipocyte differentiation while inducing markers of epithelial gut differentiation. (A) Hic-5 mRNA levels during 3T3-L1 adipogenesis were analyzed using real-time RT-PCR. (B) 3T3-L1 preadipocytes were infected with either retrovirus expressing Hic-5 or an empty retrovirus. Cells were then differentiated using a standard protocol with addition of rosiglitazone. After 4 d cells were fixed, and phase-contrast pictures were taken (top panel) or stained for lipid droplet formation by Oil-Red-O (bottom panel). (C) 3T3-L1 preadipocytes were treated as described in B. After 4 d of differentiation with or without rosiglitazone, cells were harvested and RNA was extracted. Levels of mRNA for adipocyte markers adipsin, acrp30, and aP2 and gut epithelial markers KLF4 L-FABP and keratin 20 were determined by real-time quantitative RT-PCR. Statistical analysis was done using Student's t-test. (★) p < 0.05. (D) Illustration of Hic-5 and PPARγ combination in epithelial differentiation.