Figure 6.

Comparison of predicted amino-acid sequence of cDNAs and paralogous genomic copies of double homeobox DUX-like genes. DUX1, DUX2, DUX3, DUX4, DUX5, and DUX10 represent human double homeobox-containing genes from 4q35. All genes consist only of a single exon. The color code corresponds to the CLUSTALW default for amino-acid sequence comparison. The boxes indicate the 60-amino-acid conserved homeodomain. Analogous to the DUX gene family member DUX2, none of the Y-derived DUX family members contain a complete copy of the second homeodomain (Homeobox II). The location of a 1-bp deletion in DUXY1 relative to all other family members is indicated, resulting in a frameshift and a C-terminally altered amino acid sequence (purple). We resequenced the DUXY1 copy from a PCR product amplified from BAC RP11-886I11 (AC134882) and a normal male individual and confirmed the accuracy of the 1-bp deletion. Black stars indicate stop codons.