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. 2005 Feb 1;19(3):397–410. doi: 10.1101/gad.330105

Figure 6.

Figure 6.

EphrinB2 is expressed predominantly in the endothelial cells of collecting lymphatic vessels, while EphB4 is in both lymphatic capillaries and collecting lymphatic vessels. Triple immunofluorescence for LYVE-1, podoplanin, and αSMA (A-D); for ephrinB2 (βgal), podoplanin, and αSMA (E-H); and for EphB4, podoplanin, and LYVE-1 (I-L) of the ear skin at P21. (A-D) High LYVE-1 expression is detected in the lymphatic capillaries (arrow) but not in the αSMA-positive collecting lymphatic vessels (arrowhead). Podoplanin instead stains all lymphatic vessels. αSMA also stains blood vessels (bv). (E-H) EphrinB2 is expressed on the endothelium of collecting lymphatic vessels (αSMA-positive, arrowhead), but not on podoplanin-positive, αSMA-negative lymphatic capillaries (arrow). (I-L) EphB4 is expressed both on the LYVE-1-positive lymphatic capillaries (arrow) and on the LYVE-1-negative collecting lymphatic vessels (arrowhead). In addition, the veins are positive for EphB4. Bars, 100 μm.