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. 2017 Jun 8;7:3038. doi: 10.1038/s41598-017-03366-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequential fluorescent labeling of bone formation and mineralization. (A) Red, green and yellow represent labels of Alizarin Red S (AL), Calcein (CA) and Tetracycline Hydrochloride (TE) respectively for each group and merged images of the three fluorochromes for the same group (Original magnification x100). (B) The graph shows the percentage of the three fluorochromes area in each group. (^***p < 0.001vs CO, ^##p < 0.01, ^###p < 0.001vs EPC, ^&p < 0.05, ^&&&p < 0.001 vs BMSC).