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. 2017 Jun 8;7:3043. doi: 10.1038/s41598-017-02934-3

Figure 2.

Figure 2

The inhibition of NFAT reporter activity and the production of IL2. (a,b) NFAT (a) and NF-κB (b) reporter activity. Jurkat cells were electroporated with 5 μg of pNFAT-SEAP or pNF-κB-SEAP, respectively. The cells were incubated with 1 μM FK506 or 20 μM RCAN-11R/scRCAN-11R for 1 h, and stimulated with 200 nM PMA and 4 μM ionomycin for 12 h. (c) The inhibition of IL-2 transcription. Jurkat cells were treated with 1 μM FK506 or 20 μM RCAN-11R/scRCAN-11R for 1 h, then with 200 nM PMA and 4 μM ionomycin for an additional 12 h. The cells were subjected to a quantitative RT-PCR. (d) The inhibition of IL-2 production. Jurkat cells were treated with 1 μM FK506 or 20 μM RCAN-11R/scRCAN-11R for 1 h and then incubated with 200 nM PMA and 4 μM ionomycin for an additional 12 h. *p < 0.01 in comparison to stimulation (control) without treatment of FK506 or peptides. The values represent the mean ± SE of five independent experiments.