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. 2017 Jun 8;7:3036. doi: 10.1038/s41598-017-02785-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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An efficient and versatile electroporation system. (A) Transient transfection of the pEGFP-N1 vectors into the PFFs. The transfection efficiency was analysed with fluorescence microscopy and FACS at 24 hours post-transfection. (B) Transient transfection of the pEGFP-N1 vector into the indicated cell lines. EGFP fluorescence was analysed via fluorescence microscopy. (C) Transfection of the EGFP mRNA and CY3-labelled siRNA into PFFs. The fluorescence was analysed via fluorescence microscopy.