Figure 7.

Effect of Puf1 and Puf2 double deletion. (a) Heatmap of SGD GO enrichment analysis of significantly affected genes in T₄₅/T₀ and T_end/T₀ in puf1∆puf2∆ relative to wild-type α. The red or blue shades indicate levels of enrichment score of induced or repressed respectively, and the color intensity indicates the level of p value enrichment score of each GO term. Only primary GO terms with p value < 10e⁻⁴ and FDR ≤ 0.01 are presented. (b) Scatterplots of log₂ normalized reads of puf1Δpuf2Δ/WT α against multiplication of puf1Δ/WT α and puf2Δ/WT ‘a’ in T₄₅/T₀ (I) and T_end/T₀ (II). Genes significantly affected by the synergism of Puf1 and Puf2 knockouts are colored in red (two standard deviations above the trend line) or in blue (two standard deviations below the trend line). Genes bound by Puf1 or Puf2^{13, 15} within the two standard deviations areas are colored in green.