Figure 1.

Composition of major T lymphocyte subpopulations in visceral adipose tissue (VAT) stromal vascular fraction (SVF) versus peripheral blood (PB) of lean healthy subjects. Cell subset percentages within total PB and VAT SVF lymphocytes of nine healthy lean subjects were analyzed. Gating strategies for NK, NKT, T, and γδ T cells (A), total regulatory T (T_reg), conventional T cells (T_conv), OX40⁺ T_reg and activated T_reg cells (aT_reg) (F), in PB and SVF from a representative donor. γδ T cells, TCRγδ⁺CD3⁺ (B); NK cells, CD3⁻CD56⁺ (D,E); NKT cells, CD3⁺CD56⁺ (C); T_reg cells, CD4⁺FOXP3⁺CD127^low (G); aT_reg, FOXP3^highCD45RA⁻ (H); OX40⁺ T_reg cells (I). Each dot represents an individual donor. Mean ± SEM is shown for each group. *p < 0.05, **p < 0.01, by unpaired Student’s t-test.