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. 2017 Jun 9;8:643. doi: 10.3389/fimmu.2017.00643

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Copyright © 2017 Donninelli, Del Cornò, Pierdominici, Scazzocchio, Varì, Varano, Pacella, Piconese, Barnaba, D’Archivio, Masella, Conti and Gessani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The frequency of visceral adipose tissue (VAT)-associated NKT cells, but not NK cells, is increased in colorectal cancer (CRC)-affected subjects as compared to cancer-free donors. VAT stromal vascular fraction (SVF) and peripheral blood (PB) lymphocytes isolated from lean normal weight (Nw), obese (Ob), lean affected by CRC (Nw/CC), and Ob affected by CRC (Ob/CC) donors were analyzed by flow cytometry. Frequencies of NKT (CD3⁺CD56⁺) (A) and NK (CD3⁻CD56⁺) (B) cells were evaluated in the four categories of donors. Each dot represents an individual donor. Mean ± SEM is shown for each group. *p < 0.05, by analysis of variance.

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