Table 2.
Culture and screening tetraplex real-time PCR results of positive samples (detection of M. avium, M. tuberculosis complex and genus Mycobacterium).
| Product | From | Sample ID | Product culture (isolation medium)a | Product direct PCR (CT)a | Culture PCR (CT)a |
|---|---|---|---|---|---|
| Pasteurized whole milk | Cattle | 1 | N | Mycobacterium (32.64) | ND |
| Whole milk powder | Cattle | 2 | N | Mycobacterium (43.62) | N |
| Pasteurized milk fresh cheese | Cattle | 3 | N | Mycobacterium (37.62) | N |
| Pasteurized milk fresh cheese | Cattle | 4 | N | Mycobacterium (36.42) | Mycobacterium (42.28) |
| Pasteurized milk fresh cheese | Cattle | 5 | N | Mycobacterium (38.31) | ND |
| Pasteurized milk fresh cheese | Cattle | 6 | N | Mycobacterium (38.98) | Mycobacterium (41.64) |
| Pasteurized milk soft cheese | Cattle | 7 | N | Mycobacterium (37.21) | ND |
| Pasteurized milk greek style yogurt | Cattle | 8 | N | Mycobacterium (38.46) | ND |
| Mortadella cold cut | Pig | 9 | P (MGIT) | N | Mycobacterium (25.6) |
| Pasteurized whole milk | Cattle | 10 | N | M. avium (39.1) | ND |
| Powdered infant formula | Cattle | 11 | N | M. avium (36.79) | M. avium (42.01) |
| Powdered infant formula | Cattle | 12 | P (M7H10) | N | M. avium (25.14) |
| UHT milk cream | Cattle | 13 | N | M. avium (38.77) | ND |
| Pasteurized milk fresh cheese | Cattle | 14 | N | M. avium (37.8) | ND |
| Pasteurized milk fresh cheese | Cattle | 15 | N | M. avium (36.74) | ND |
| Pasteurized milk fresh cheese | Cattle | 16 | N | M. avium (38.77) | N |
| Ground meat | Cattle | 17 | P (M7H10) | N | M. avium (14.34) |
| Hamburger | Horse | 18 | N | M. avium (37) | N |
| Fresh sausage | Chicken | 19 | N | M. avium (39) | N |
| Fresh sausage | Chicken | 20 | P (MGIT) | N | M. avium (14.90) |
| Whole milk powder | Cattle | 21 | N | M. tuberculosis complex (40) | N |
| Powdered infant formula | Cattle | 22 | N | M. tuberculosis complex (38.29) | N |
| Butter | Cattle | 23 | N | M. tuberculosis complex (40.03) | ND |
| Butter | Cattle | 24 | N | M. tuberculosis complex (38.08) | ND |
| Pasteurized milk fresh cheese | Cattle | 25 | N | M. tuberculosis complex (38.25) | N |
| Pasteurized milk soft cheese | Cattle | 26 | N | M. tuberculosis complex (40.12) | N |
| Pasteurized milk greek style yogurt | Cattle | 27 | N | M. tuberculosis complex (39.86) | M. tuberculosis complex (38.45) |
a
P, positive; N, negative; ND, not done; CT, threshold cycle; M7H10, modified Middlebrook 7H10; MGIT, Mycobacterial Growth Indicator Tube.